Synthesis of type II collagen in vitro by embryonic chick neural retina tissue.

  title={Synthesis of type II collagen in vitro by embryonic chick neural retina tissue.},
  author={Gerald N. Smith and Thomas F. Linsenmayer and David A. Newsome},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={73 12},
Collagen produced by chick neural retina tissue in vitro has the properties of type II collagen. Isolated neural retina tissue from stage 29-32 chick embryos incorporated [14C]glycine and [3H]proline into collagen consisting of alpha1 chains and a higher molecular weight species. The peptides produced by cyanogen bromide digestion of the collagen are identical to those from authentic type II collagen from cartilage in charge properties, as determined by carboxymethyl-cellulose chromatography… CONTINUE READING

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