Synthesis of the Pitstop family of clathrin inhibitors

@article{Robertson2014SynthesisOT,
  title={Synthesis of the Pitstop family of clathrin inhibitors},
  author={Mark J. Robertson and Fiona M. Deane and Wiebke Stahlschmidt and Lisa von Kleist and Volker Haucke and Phillip J Robinson and Adam McCluskey},
  journal={Nature Protocols},
  year={2014},
  volume={9},
  pages={1592-1606}
}
This protocol describes the synthesis of two classes of clathrin inhibitors, Pitstop 1 and Pitstop 2, along with two inactive analogs that can be used as negative controls (Pitstop inactive controls, Pitnot-2 and Pitnot-2-100). Pitstop-induced inhibition of clathrin TD function acutely interferes with clathrin-mediated endocytosis (CME), synaptic vesicle recycling and cellular entry of HIV, whereas clathrin-independent internalization pathways and secretory traffic proceed unperturbed; these… CONTINUE READING
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