Synthesis of regioselectively protected forms of cytidine based on enzyme-catalyzed deacetylation as the key step.

Abstract

N4-Acetylcytidine (77%) and 2',3'-O, N4-triacetylcytidine (95%) were obtained from the hydrolysis of a common precursor, the peracetylated form of cytidine with Aspergillus niger lipase (Amano A) and Burkholderia cepacia esterase (SC esterase S), respectively, under very mild conditions. The experimental procedure for the conversion of triacetylcytidine to a corresponding phosphoramidite (82%), an intermediate for sugar nucleotide synthesis, is also elaborated.

Cite this paper

@article{Kuboki2000SynthesisOR, title={Synthesis of regioselectively protected forms of cytidine based on enzyme-catalyzed deacetylation as the key step.}, author={Atsuhito Kuboki and Takeshi Ishihara and Eiji Kobayashi and Hitoya Ohta and Tetsuro Ishii and A. Inoue and Satoshi Mitsuda and Takuro Miyazaki and Yasumasa Kajihara and Tamotsu Sugai}, journal={Bioscience, biotechnology, and biochemistry}, year={2000}, volume={64 2}, pages={363-8} }