Synthesis of protein‐containing polymers in organic solvents

@article{Yang1995SynthesisOP,
  title={Synthesis of protein‐containing polymers in organic solvents},
  author={Z. Yang and D. Williams and Alan J. Russell},
  journal={Biotechnology and Bioengineering},
  year={1995},
  volume={45}
}
Subtilisin has been modified with polyethylene glycol (PEG) monomethacrylate (MW 8000) by reductive alkylation, and incorporated into polymethyl methacrylate durring free‐radical initiated polymerization. The activity and stability of the PEG‐modified enzymes have been determined in aqueous buffer and organic solvents. The Km and Vmax values for unmodified, singly and doubly modified subtilisin were compared in these environments, and the half‐lives of both modified enzymes were remarkably high… 
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Copolymers of acrylated derivatives of alpha-chymotrypsin and polyethylene glycol (PEG) have been prepared and used as biocatalysts for the synthesis of model peptides in organic solvent containing a
Lipase Modified for Solubility in Organic Solvents
TLDR
Les proteines modifiees par le PEG sont par immunogenes et ont une duree de vie allongee, fixant ainsi sur la lipase de Candida rugosa, des molecules de PEG de longueur differente.
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This is the second edition of this biological reference aimed at undergraduates and graduates. The book covers the structure and mechanism of enzymes, creating a guide to the current understanding of