Synthesis of pentadecanncleotide duplex containing the ideal Pribnow sequence of promoter by tbe phosphotriester method using a new phosphorylating reagent

Abstract

A phosphorylating reagent o-chlorophenyl phosphoro-p-anisidochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides… (More)

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