Synthesis of oxalyl phosphate and processing of the acyl phosphate by phosphoenolpyruvate-dependent enzymes.

@article{Kofron1990SynthesisOO,
  title={Synthesis of oxalyl phosphate and processing of the acyl phosphate by phosphoenolpyruvate-dependent enzymes.},
  author={J. Kofron and G. H. Reed},
  journal={Archives of biochemistry and biophysics},
  year={1990},
  volume={280 1},
  pages={
          40-4
        }
}
  • J. Kofron, G. H. Reed
  • Published 1990
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • The mixed anhydride of oxalic and phosphoric acids, oxalyl phosphate, has been prepared by reaction of oxalyl chloride and inorganic phosphate in aqueous solution. The product was purified by anion exchange chromatography and characterized by 31P and 13C NMR. This acyl phosphate has a half-life of 51 h at pH 5.0 and 4 degrees C. Oxalyl phosphate, an analogue of phosphoenolpyruvate, is a slow substrate for pyruvate kinase, undergoing an enzyme-dependent phosphotransfer reaction to produce ATP… CONTINUE READING
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