A new series of aliphatic thiourea and various aryl urea incorporating 1,3,5-s-triazine moiety is reported. This series has been obtained by the reaction of cyanuric chloride with thiophene-2-ethyl thiourea 1. Thus, the prepared 2-(thiophene-2ethyl thioureido)-4,6-dichloro-s-triazine 2 has been subsequently treated with morpholine to get 2-(thiophene-2-ethyl thioureido)-4-(morpholino)-6-chloro-s-triazine 3. This is further treated with various aryl ureas to afford title compound 4a-j. The library of ureido linkage containing triazinyl moiety has been tested for in vitro antibacterial and antifungal activity against different microorganisms. The structure of novel synthesized compounds has been established on the basis of elemental analysis, H NMR, IR and mass spectral data.