Synthesis of mucin glycoproteins by epithelial cells isolated from swine trachea by specific proteolysis


Mucus-producing cells were isolated from swine trachea mucosa by a method that included enzymatic digestion of the epithelial surface with Dispase, a neutral protease fromBacillus polymyxa, and differential attachment of the washed cells to culture flasks coated with collagen. Epithelial cells were the major cell type isolated by these procedures. Ciliated… (More)
DOI: 10.1007/BF02619589


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