Synthesis of linear gramicidin requires the cooperation of two independent reductases.

@article{Schracke2005SynthesisOL,
  title={Synthesis of linear gramicidin requires the cooperation of two independent reductases.},
  author={Nadine Schracke and Uwe Linne and Christoph Mahlert and Mohamed A. Marahiel},
  journal={Biochemistry},
  year={2005},
  volume={44 23},
  pages={8507-13}
}
The linear pentadecapeptide gramicidin has been reported to be assembled by four large multimodular nonribosomal peptide synthetases (NRPSs), LgrABCD, that comprise 16 modules. During biosynthesis, the N-formylated 16mer peptide is bound to the peptidyl carrier protein (PCP) of the terminal module via a thioester bond to the carboxyl group of the last amino acid glycine(16). In a first reaction the peptide is released from the protein template in an NAD(P)H-dependent reduction step catalyzed by… CONTINUE READING