Synthesis of l- and d-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization.


Native chemical ligation combined with desulfurization has become a powerful strategy for the chemical synthesis of proteins. Here we describe the use of a new thiol additive, methyl thioglycolate, to accomplish one-pot native chemical ligation and metal-free desulfurization for chemical protein synthesis. This one-pot strategy was used to prepare ubiquitin from two or three peptide segments. Circular dichroism spectroscopy and racemic protein X-ray crystallography confirmed the correct folding of ubiquitin. Our results demonstrate that proteins synthesized chemically by streamlined 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis coupled with a one-pot ligation-desulfurization strategy can supply useful molecules with sufficient purity for crystallographic studies.

DOI: 10.1002/chem.201600101

Cite this paper

@article{Huang2016SynthesisOL, title={Synthesis of l- and d-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization.}, author={Yi-Chao Huang and Chen-Chen Chen and Shuai Gao and Ye-Hai Wang and Hua Xiao and Feng Wang and Changlin Tian and Yi-Ming Li}, journal={Chemistry}, year={2016}, volume={22 22}, pages={7623-8} }