Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region.

@article{Laub1983SynthesisOH,
  title={Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region.},
  author={O Laub and L. B. Rall and Martha A. Truett and Yosef Shaul and David N. Standring and Pablo Valenzuela and William J. Rutter},
  journal={Journal of virology},
  year={1983},
  volume={48 1},
  pages={271-80}
}
We have constructed two simian virus 40 early replacement recombinants that have the coding sequences for hepatitis B virus surface antigen (HBsAg). One construction, LSV-HBsAg, has the coding region for HBsAg but not the portion encoding the putative pre-surface antigen leader. Transformed monkey kidney cells (COS) infected with this recombinant express large quantities of the characteristic partially glycosylated HBsAg molecule, which are assembled into 22-nm particles that appear similar to… CONTINUE READING