Synthesis of d1-N-ethyltramadol as an internal standard for the quantitative determination of tramadol in human plasma by gas chromatography-mass spectrometry.

@article{Leis2004SynthesisOD,
  title={Synthesis of d1-N-ethyltramadol as an internal standard for the quantitative determination of tramadol in human plasma by gas chromatography-mass spectrometry.},
  author={Hans J{\"o}rg Leis and G{\"u}nter Fauler and Werner Windischhofer},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2004},
  volume={804 2},
  pages={
          369-74
        }
}
  • H. Leis, G. Fauler, W. Windischhofer
  • Published 25 May 2004
  • Chemistry, Medicine
  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
A gas chromatography-mass spectrometry (GC-MS) assay for the determination of tramadol in human plasma is presented. The synthesis of an N-ethyl analogue of the drug is described and its use as an internal standard for the quantitative measurement of tramadol in human plasma is described. The method involves extraction at plasma pH and analysis of the underivatized drug by gas chromatography-electron ionization mass spectrometry using m/z 58 and 73 for detection of tramadol and internal… 
Enatiomeric determination of tramadol and O-desmethyltramadol in human urine by gas chromatography-mass spectrometry.
TLDR
This GC-MS assay for stereoselective determination of tramadol and its pharmacologically active phase I metabolite O-desmethyltramadol in human urine was developed and was successfully used to determine the concentration of enantiomers of T and ODT in a pharmacogenetic study.
An accurate, rapid and sensitive determination of tramadol and its active metabolite O-desmethyltramadol in human plasma by LC-MS/MS.
TLDR
The method was fully validated for its selectivity, sensitivity, linearity, precision and accuracy, recovery, matrix effect, ion suppression/enhancement, stability and dilution integrity and successfully applied to a bioequivalence study of 200mg tramadol tablet formulation in 27 healthy Indian male subjects under fasting condition.
Simultaneous determination of tramadol, O-desmethyltramadol and N-desmethyltramadol in human urine by gas chromatography-mass spectrometry.
TLDR
This method detected urine concentrations of T, ODT and NDT in six healthy volunteers for 7 days after administration of 50 mg oral doses of tramadol.
Simultaneous determination of tramadol and O-desmethyltramadol in human plasma using HPLC-DAD.
TLDR
The method was successfully applied to quantify T and ODT from authentic plasma samples received from Hospital Sohag University and can be of interest to clinical and forensic laboratories.
Rapid determination of tramadol in human plasma by headspace solid-phase microextraction and capillary gas chromatography-mass spectrometry.
TLDR
The proposed method was successfully applied to determination of tramadol in human plasma samples from 10 healthy volunteers after a single oral administration and showed linearity in the range of 1-400 ng mL(-1).
Simultaneous Determination of Tramadol and Acetaminophen in Human Plasma by LC–ESI–MS
This study presents a high-performance liquid chromatography–electrospray ionization–mass spectrometric (LC–ESI–MS) method for the simultaneous determination of tramadol and acetaminophen in human
Simultaneous determination of ketamine, tramadol, methadone, and their metabolites in urine by gas chromatography-mass spectrometry.
TLDR
Results have demonstrated that this GC-MS method is a good confirmation and quantitation test scheme for the six target compounds in urine.
Determination of tramadol by dispersive liquid-liquid microextraction combined with GC-MS.
TLDR
Under optimal conditions and by using 2-nitro phenol as internal standard, tramadol was determined by GC-MS, and the figures of merit of this work were evaluated.
Development and Validation of High-Performance Liquid Chromatography - Diode Array Detector Method for the Determination of Tramadol in Human Saliva
A high-performance liquid chromatography–diode array detector method (HPLC-DAD) was developed and validated for the determination of tramadol in human saliva. Samples of saliva were prepared
Determination of tramadol in human plasma and urine samples using liquid phase microextraction with back extraction combined with high performance liquid chromatography.
TLDR
The method was successfully applied to determine the concentration of tramadol in the plasma and urine samples and satisfactory results were obtained.
...
1
2
3
...

References

SHOWING 1-10 OF 18 REFERENCES
Determination of tramadol in human plasma by capillary gas chromatography-mass spectrometry using solid-phase extraction.
An analytical method using solid-phase extraction, capillary gas chromatography and mass selective detection in the electron-impact ionization (EI) mode was developed for the determination of
Simultaneous determination of tramadol and its major active metabolite O-demethyltramadol by high-performance liquid chromatography with electrochemical detection.
TLDR
A novel, highly sensitive method was developed for simultaneous determination of tramadol and its main active metabolite O-demethyltramadol in rat plasma, which allows an adequate characterization of the plasma vs. time profiles for both compounds.
Quantitative determination of tramadol in human serum by gas chromatography-mass spectrometry.
TLDR
The gas chromatographic-mass spectrometric method for the quantitative determination of tramadol in human serum, plasma or whole blood samples is described, and it is specific, sensitive and precise, and has high accuracy.
Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d1-N-ethylclindamycin as internal standard.
TLDR
A new method for the quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS) is presented, which was validated in the expected concentration range for a pharmacokinetic study and is simple and robust.
Enantiomeric determination of tramadol and its main metabolite O-desmethyltramadol in human plasma by liquid chromatography-tandem mass spectrometry.
TLDR
The method developed is based on on-line LC-MS-MS using atmospheric pressure chemical ionization as an interface determination of enantiomers of tramadol and its active metabolite O-desmethyltramadol in human plasma and was successfully used to investigate plasma concentration of enantomers of T and ODT in a pharmacokinetic study.
High-performance liquid chromatographic assay for simultaneous determination of tramadol and its active metabolite in human plasma. Application to pharmacokinetic studies
SummaryA sensitive liquid chromatographic assay for the quantitative determination of the opioid analgesic tramadol and its active metabolite is described. Fluconazole was used as internal standard.
Gas chromatographic method using nitrogen-phosphorus detection for the measurement of tramadol and its O-desmethyl metabolite in plasma and brain tissue of mice and rats.
  • Q. Tao, D. Stone, +6 authors R. Raffa
  • Chemistry, Medicine
    Journal of chromatography. B, Biomedical sciences and applications
  • 2001
A method that allows the measurement of plasma and brain levels of the centrally-acting analgesic tramadol and its major metabolite (O-desmethyl tramadol) in mice and rats was developed using gas
Simultaneous stereoselective analysis by capillary electrophoresis of tramadol enantiomers and their main phase I metabolites in urine.
Capillary zone electrophoresis was successfully applied to the enantiomeric resolution of racemic tramadol and its six phase I metabolites using carboxymethylated beta-cyclodextrin (CMB) added to the
High-performance liquid chromatographic determination of tramadol in human plasma.
TLDR
Tramadol has been determined in human plasma samples using a sensitive high-performance liquid chromatographic method and analyses of the extracts were performed on reversed-phase silica gel using ion-pair chromatography and fluorescence detection.
Determination of common drugs of abuse in body fluids using one isolation procedure and liquid chromatography--atmospheric-pressure chemical-ionization mass spectromery.
TLDR
The extraction method appeared very universal; the recoveries were high for almost all drugs and the extracts were very clean; the procedure was applied for routine forensic casework.
...
1
2
...