Synthesis of chimeric mouse nerve growth factor precursor and human beta-nerve growth factor in Escherichia coli: immunological properties.

Abstract

The complete mouse prepro-nerve growth factor (NGF) DNA was fused to the carboxyl terminus of the beta-galactosidase (lac-z) gene of Escherichia coli. Similarly, a genomic fragment encoding the human NGF comprising codons 11 to 106 (from a total of 118) was fused to the fifth codon of the amino terminus of beta-galactosidase. Both bacterial vectors produce… (More)

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@article{Dicou1989SynthesisOC, title={Synthesis of chimeric mouse nerve growth factor precursor and human beta-nerve growth factor in Escherichia coli: immunological properties.}, author={Eleni Dicou and R{\'e}mi Houlgatte and Jonathan Lee and B von Wilcken-Bergmann}, journal={Journal of neuroscience research}, year={1989}, volume={22 1}, pages={13-9} }