Synthesis of biologically active influenza virus core proteins using a vaccinia virus-T7 RNA polymerase expression system.

@article{Mena1994SynthesisOB,
  title={Synthesis of biologically active influenza virus core proteins using a vaccinia virus-T7 RNA polymerase expression system.},
  author={I. Mena and S. de la Luna and C. Albo and J. Mart{\'i}n and A. Nieto and J. Ort{\'i}n and A. Portela},
  journal={The Journal of general virology},
  year={1994},
  volume={75 ( Pt 8)},
  pages={
          2109-14
        }
}
  • I. Mena, S. de la Luna, +4 authors A. Portela
  • Published 1994
  • Biology, Medicine
  • The Journal of general virology
  • An in vivo system in which expression of a synthetic influenza virus-like chloramphenicol acetyltransferase (CAT) RNA is driven by influenza virus proteins synthesized from cloned cDNAs has been developed. Expression of the four influenza virus core proteins (nucleoprotein, PA, PB1 and PB2) was performed by transfection of four pGEM recombinant plasmids, each containing one of the four viral genes, into cell cultures previously infected with a vaccinia virus recombinant encoding the T7 RNA… CONTINUE READING
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