Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability.

@article{GrudzienNogalska2007SynthesisOA,
  title={Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability.},
  author={Ewa Grudzien-Nogalska and Janusz Stępiński and Jacek Jemielity and Joanna Żuberek and Ryszard Stolarski and R. E. Rhoads and Edward Darzynkiewicz},
  journal={Methods in enzymology},
  year={2007},
  volume={431},
  pages={203-27}
}
Synthetic capped RNA transcripts produced by in vitro transcription in the presence of m(7)Gp(3)G have found a wide application in studying such processes as mRNA translation, pre-mRNA splicing, mRNA turnover, and intracellular transport of mRNA and snRNA. However, because of the presence of a 3'-OH on both m(7)Guo and Guo moieties of the cap structure, one… CONTINUE READING