Synthesis of amidrazones using an engineered papain nitrile hydratase.

@article{Dufour1998SynthesisOA,
  title={Synthesis of amidrazones using an engineered papain nitrile hydratase.},
  author={Evelyne Dufour and W Vanessa Tam and Dorit Karin N{\"a}gler and A. C. Storer and Robert M{\'e}nard},
  journal={FEBS letters},
  year={1998},
  volume={433 1-2},
  pages={78-82}
}
To demonstrate the usefulness of an engineered papain nitrile hydratase as a biocatalyst, a peptide amidrazone was prepared by incubation of the nitrile MeOCO-Phe-Alanitrile with the Gln19Glu papain mutant in the presence of salicylic hydrazide as a nucleophile. The amidrazone results from nucleophilic attack by salicylic hydrazide at the imino carbon of… CONTINUE READING