Synthesis of alpha-galactooligosaccharides with alpha-galactosidase from Lactobacillus reuteri of canine origin.

@article{Tzortzis2003SynthesisOA,
  title={Synthesis of alpha-galactooligosaccharides with alpha-galactosidase from Lactobacillus reuteri of canine origin.},
  author={George Tzortzis and A. J. Jay and M L A Baillon and Glenn R M Gibson and R. A. Rastall},
  journal={Applied microbiology and biotechnology},
  year={2003},
  volume={63 3},
  pages={286-92}
}
Crude cell-free extracts from Lactobacillus reuteri grown on cellobiose, maltose, lactose and raffinose were assayed for glycosidic activities. When raffinose was used as the carbon source, alpha-galactosidase was produced, showing the highest yield at the beginning of the stationary growth phase. A 64 kDa enzyme was purified by ultra- and gel filtration, and characterized for its hydrolytic and synthetic activity. Highest hydrolytic activity was found at pH 5.0 at 50 degrees C ( K(M) 0.55 mM… CONTINUE READING