Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.

Abstract

A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for… (More)

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@article{Sheppeck2000SynthesisOA, title={Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.}, author={James E. Sheppeck and Haranath Kar and Luke J. Gosink and Jeffrey Wheatley and Erik Gjerstad and Shawn Loftus and A R Zubiria and Jarosław Janc}, journal={Bioorganic & medicinal chemistry letters}, year={2000}, volume={10 23}, pages={2639-42} }