Corpus ID: 22334500

Synthesis of a new organic pyrophosphate in large quantities is induced in some bacteria by oxidative stress.

  title={Synthesis of a new organic pyrophosphate in large quantities is induced in some bacteria by oxidative stress.},
  author={D. Ostrovsky and E. Kharatian and I. Malarova and I. Shipanova and L. Sibeldina and A. Shashkov and G. Tantsirev},
  volume={3 4},
Brevibacterium ammoniagenes and Micrococcus luteus were shown to synthesize up to 50 mM of a novel substance, 2-methylbutan-1,2,3,4-tetraol 2,4-cyclopyrophosphate, in response to oxidative stress created by benzyl viologen and other redox mediators under aerobic conditions. The substance, which represents greater than 50% of the extractable phosphorus, is suggested to play a role as a bacterial antistressor and is thought to be a product of condensation of two molecules of phosphoenolpyruvate… Expand
Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria
A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed. Expand
Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
Abstract A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl- d -erythritol 2,4-cyclodiphosphate (Expand
Biosynthesis of 2-C-methyl-D-erythritol, a putative C-5 intermediate in the mevalonate independent pathway for isoprenoid biosynthesis
According to incorporations of 13 labelled glucose, 2-C-methyl-d-erythritol was most likely synthesized by Corynebacterium ammoniagenes from glyceraldehyde 3-phosphate and from pyruvate via aExpand
Isoprenoid biosynthesis as a novel target for antibacterial and antiparasitic drugs.
The mevalonate-independent methylerythritol phosphate pathway is a long overlooked metabolic pathway for isoprenoid biosynthesis that is present in most bacteria, including pathogens and opportunistic pathogens, in some unicellular eukaryotes, and in the chloroplasts of all phototrophic organisms. Expand
Isoprenoid biosynthesis via the methylerythritol phosphate pathway: accumulation of 2- C -methyl- d -erythritol 2,4-cyclodiphosphate in a gcpE deficient mutant of Escherichia coli
Abstract In the bacterium Escherichia coli , gcpE is an essential gene in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Incubation of [1- 3 H]methylerythritol with an E . coliExpand
Isoprenoid biosynthesis via the mevalonate-independent route, a novel target for antibacterial drugs?
  • M. Rohmer
  • Chemistry, Medicine
  • Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques
  • 1998
2C-Methyl-D-erythritol was shown to be a putative intermediate in the biosynthetic pathway leading to isopentenyl diphosphate, which potentially represents a novel target for antibacterial drugs and herbicides. Expand
The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms.
Recent studies have uncovered the existence of an alternative, non-mevalonate pathway for the formation of isopentenyl pyrophosphate and dimethylallyl pyrophosphate, the two building blocks ofExpand
Electron-dense granules in Desulfovibrio gigas do not consist of inorganic triphosphate but of a glucose pentakis(diphosphate).
Using multinuclear NMR spectroscopy it was shown that the electron-dense bodies of D. gigas contained a novel metabolite which was identified as alpha-glucose 1,2,3,4,6-pentakis(diphosphate), which did not consist of polyphosphates. Expand
Synthesis of 2-C-methyl-D-erythritol 2,4-cyclopyrophosphate.
The synthesis of 2-C-methyl-D-erythritol 2,4-cyclopyrophosphate, a biochemical intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis, was accomplished in four steps. Expand
Structure and Function of Four Classes of the 4Fe-4S Protein, IspH.
It is shown, for the first time, that there are multiple IspH classes that have evolved to allow organisms to survive in diverse oxidative-stress environments. Expand