Synthesis of a gene for the HIV transactivator protein TAT by a novel single stranded approach involving in vivo gap repair.


The synthesis of a gene for the HIV TAT protein is described using a novel approach that capitalises on the ability to synthesise oligonucleotides of greater than 100 bp in length. It involves the synthesis of large oligomers covering one strand of the desired gene in its entirety and the use of small complementary bridging and adapter oligonucleotides to… (More)

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