Synthesis of Y chromosome-specific labeled DNA probes by in vitro DNA amplification.

@article{Weier1990SynthesisOY,
  title={Synthesis of Y chromosome-specific labeled DNA probes by in vitro DNA amplification.},
  author={H U Weier and Richard Segraves and Daniel Pinkel and Joe W. Gray},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1990},
  volume={38 3},
  pages={421-6}
}
We describe the use of in vitro DNA amplification for production of double-stranded, biotin-labeled DNA probes. Specifically, a 124 BP DNA segment of the Y chromosome-specific 3.4 KB repeat was amplified in preparations of human genomic DNA using the polymerase chain reaction (PCR) and a thermostable DNA polymerase. The PCR products were amplified further in the presence of a molar excess of biotin-11-dUTP. The resulting double-stranded DNA segments showed a high amount of incorporated biotin… CONTINUE READING