Synthesis of Seoul virus RNA and structural proteins in cultured cells

@article{Kariwa2003SynthesisOS,
  title={Synthesis of Seoul virus RNA and structural proteins in cultured cells},
  author={Hiroaki Kariwa and Hideyuki Tanabe and Tetsuya Mizutani and Y. Kon and Kumari Lokugamage and Nandadeva Lokugamage and Masahiro A. Iwasa and Tomohiro Hagiya and Koichi Araki and K Yoshimatsu and Jiro Arikawa and Ikuo Takashima},
  journal={Archives of Virology},
  year={2003},
  volume={148},
  pages={1671-1685}
}
Summary.  Seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). The virion has a tripartite (S, M, and L) negative-stranded RNA genome, which is characteristic of the family Bunyaviridae. However, the molecular basis of virus replication is not well known. We established a Northern blot hybridization (NB) procedure using digoxygenin-labeled RNA probes, to quantitate the hantaviral plus- and minus-strand RNAs separately. Virus RNA replication was analyzed in… CONTINUE READING

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