Synthesis of Seoul virus RNA and structural proteins in cultured cells

  title={Synthesis of Seoul virus RNA and structural proteins in cultured cells},
  author={Hiroaki Kariwa and Hitomi Tanabe and Tetsuya Mizutani and Yasuhiro Kon and Kumari G. Lokugamage and Nandadeva Lokugamage and Masahiro Iwasa and Tomohiro Hagiya and Koichi Araki and Kumiko Yoshimatsu and Jiro Arikawa and Ikuo Takashima},
  journal={Archives of Virology},
Summary.  Seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). The virion has a tripartite (S, M, and L) negative-stranded RNA genome, which is characteristic of the family Bunyaviridae. However, the molecular basis of virus replication is not well known. We established a Northern blot hybridization (NB) procedure using digoxygenin-labeled RNA probes, to quantitate the hantaviral plus- and minus-strand RNAs separately. Virus RNA replication was analyzed in… 

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