Synthesis of L-3,4-dihydroxyphenylalanine by tyrosine hydroxylase cDNA-transfected C6 cells: application for intracerebral grafting.

@article{Uchida1989SynthesisOL,
  title={Synthesis of L-3,4-dihydroxyphenylalanine by tyrosine hydroxylase cDNA-transfected C6 cells: application for intracerebral grafting.},
  author={Kohsuke Uchida and Ken Takamatsu and Norio Kaneda and Shigeo Toya and Yasuzo Tsukada and Yoshikazu Kurosawa and Kazutoshi Fujita and Toshiharu Toshi Nagatsu and Shinichi Kohsaka},
  journal={Journal of neurochemistry},
  year={1989},
  volume={53 3},
  pages={728-32}
}
In the present study, we obtained genetically manipulated nonneuronal cells which synthesize a catecholamine precursor for future use in intracerebral grafting. Human type 1 tyrosine hydroxylase (TH; EC 1.14.16.2) cDNA was inserted into eukaryotic expression vector pKCRH2 and was co-transfected into C6 cells with plasmid pSV2neo. Expression of the TH minigene was screened by immunohistochemical staining with TH antibody and immunoblotting analysis. Several clones of the C6 transfectants that… CONTINUE READING