Synthesis of E colicins in Escherichia coli

  title={Synthesis of E colicins in Escherichia coli},
  author={Jenny Tyler and David J Sherratt},
  journal={Molecular and General Genetics MGG},
Mitomycin C treatment of Escherichia coli cells containing one of the ColE plasmids results in specific inhibition of chromosomal protein synthesis and a high rate of protein synthesis from about 35% of the plasmid genome, whereas similar treatment of plasmid-free cells has no measureable effect on protein synthesis. In the case of ColE2-and ColE3-containing cells, the antibiotic colicin protein (molecular weight about 78000) and two others (molecular weight about 11000 and 6000) are… CONTINUE READING