Synthesis of Cell Constituents from C2-Units by a Modified Tricarboxylic Acid Cycle

  title={Synthesis of Cell Constituents from C2-Units by a Modified Tricarboxylic Acid Cycle},
  author={H. Kornberg and H. Krebs},
Biochemical unity revisited: microbial central carbon metabolism holds new discoveries, multi-tasking pathways, and redundancies with a reason
It is explained how genetic redundancy and functional degeneracy cause the diversity observed in central metabolic pathways, such as glycolysis, autotrophic CO2 fixation, and acetyl-CoA assimilation. Expand
PAK4 Phosphorylates Fumarase and Blocks TGFβ-Induced Cell Growth Arrest in Lung Cancer Cells.
The findings uncover an uncharacterized mechanism underlying the local effect of FH on TGFβ-induced gene transcription, on which the inhibitory effect from PAK4 promotes tumorigenesis in lung cancer. Expand
The Glyoxylate Shunt, 60 Years On.
The aim of this review is to assess how new knowledge is impacting understanding of flux control at the TCA cycle/glyoxylate shunt branch point in a wider range of genera, and to summarize recent findings implicating a role for the glyoxylates shunt in cellular functions other than metabolism. Expand
Comparative Analysis of Glyoxylate Cycle Key Enzyme Isocitrate Lyase from Organisms of Different Systematic Groups
The obtained data raise the question about the pathway of evolution of genes of the glyoxylate cycle key enzymes in cells of prokaryotes, plants, fungi, and nematodes in response to arising demands in carbohydrates. Expand
Overexpression of isocitrate lyase-glyoxylate bypass influence on metabolism in Aspergillus niger.
The icl over-expression and malonate addition had a significant impact on metabolism and on organic acid production profiles, and instead of an increased production of succinate and malate, a major increase in fumarate production was observed. Expand
Gene cloning of cold-adapted isocitrate lyase from a psychrophilic bacterium, Colwellia psychrerythraea, and analysis of amino acid residues involved in cold adaptation of this enzyme
The substitution of Ala214 for Ser in the C. psychrerythraea ICL introduced by point mutation resulted in the increased thermostability and lowering of the specific activity at low temperature, indicating that Ala214 is important for psychrophilic properties of this enzyme. Expand
Functional analysis and regulation of the malate synthase from Chlamydomonas reinhardtii
The data strongly support the role of acetate and light as the main regulatory effectors, and the existence of cross-talk between the two signalling pathways, in malate synthase. Expand
PTS1-independent targeting of isocitrate lyase to peroxisomes requires the PTS1 receptor Pex5p
ICL can be targeted to peroxisomes in a Pex5p-dependent but PTS1-independent fashion, which helps to clarify the contradictory published data about the requirement of the PTS1 signal for ICL targeting. Expand
La Biochimica prima della «doppia elica»
The main results obtained by the biochemical research between the years from 1930 to ’50 are briefly referred, covering a period of great technical innovations which promoted many discoveries andExpand
A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation
The capture of CO 2 by carboxylases is key to sustainable biocatalysis and a carbon-neutral bio-economy, yet currently limited to few naturally existing enzymes. Here, we developed glycolyl-CoAExpand


CO2 fixation during protein synthesis from ammonium acetate.
  • H. Kornberg
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 1956
Distribution and Formation of Isocitritase
Following the lead of Campbell, Smith and Eagles, Saz3, although he did not separate the aconitase from the pseudomonad extracts, has concluded from reaction-rates that isocitrate is the substrate and from inhibitor data and stimulation by yeast kochsaft that both organic and inorganic activators are required. Expand
Isocitritase in Escherichia coli
This communication describes this enzyme in Escherichia coli which splits isocitric acid to succinate and glyoxylate. Expand
The d-isoCitric Lyase System: the Formation of Glyoxylic and Succinic Acids from d-isoCitric Acid
DURING studies on the acetate metabolism of Penicillium chrysogenum in this Department, it was found that alcoholic extracts of acetate-grown mycelium contained considerable quantities of glutamicExpand
Oxidative pathways in a fluorescent Pseudomonas.
This chapter discusses the history of biochemistry and its applications in medicine, as well as some of the techniques used today, which were first introduced in the field more than 100 years ago. Expand
Enzymatic synthesis of citric acid. II. Crystalline condensing enzyme.