Synthesis of Adenine Nucleosides by Transglycosylation using Two Sequential Nucleoside Phosphorylase‐Based Bioreactors with On‐Line Reaction Monitoring by using HPLC

@article{Cattaneo2017SynthesisOA,
  title={Synthesis of Adenine Nucleosides by Transglycosylation using Two Sequential Nucleoside Phosphorylase‐Based Bioreactors with On‐Line Reaction Monitoring by using HPLC},
  author={Giulia Cattaneo and Marco Rabuffetti and Giovanna Speranza and Tom Kupfer and Benjamin Peters and Gabriella Massolini and Daniela Ubiali and Enrica Calleri},
  journal={ChemCatChem},
  year={2017},
  volume={9}
}
Uridine phosphorylase from Clostridium perfringens (CpUP, EC 2.4.2.3) was immobilized covalently in an aminopropylsilica monolithic column (25 mm×4.6 mm) upon functionalization with glutaraldehyde. Imino bonds that result from the reaction between the enzyme and the support were reduced chemically to afford a 66 % yield (13 mg) determined spectrophotometrically. The CpUP immobilized enzyme reactor (IMER) was connected to a silica particle‐based IMER that contained a purine nucleoside… 
13 Citations
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