Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

  title={Synthesis in E. coli of a polypeptide with human leukocyte interferon activity},
  author={Shigekazu Nagata and Hideharu Taira and Alan Hall and Lorraine Johnsrud and Michel Streuli and Josef Ecs{\"o}di and Werner Boll and Kari Cantell and Charles Weissmann},
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones. 

Human leukocyte interferon produced by E. coli is biologically active

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli, and protected squirrel monkeys from lethal encephalomyocarditis virus infection.

Structure and expression of human alpha-interferon genes.

Isolation and structure of a human fibroblast interferon gene

From the nucleotide sequence of the gene, the complete amino acid sequence of human fibroblast interferon was deduced and the protein is 166 amino acids long and is preceded by a 21-amino acid signal sequence.

Total synthesis of a human leukocyte interferon gene

A 514-base pair fragment of double-stranded DNA coding for human interferon-α1 (166 amino acid residues), and containing initiation and termination signals plus appropriate restriction enzyme sites

Expression of human fibroblast interferon gene in Escherichia coli

Antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized and processed to a size compatible with mature but unglycosylated authentic product.

Recombinant DNA Technology and Characterization of Recombinant Alpha-2 Interferon

The strain of E. coli, KMAC-43, used for the large-scale production of alpha-2 interferon was engineered at Schering by Leibowitz and his co-workers. The alpha-2 interferon gene used in this

Supply of the argU gene product allows high-level expression of recombinant human interferon-a2a in Escherichia coli

Escherichia coli BL21 (DE3) co-transformed with the gene of human interferon-α2a (IFN-α2a) and the argU gene that codes for minor tRNA Arg(AGG/AGA) produced IFN-α2a in yield of more than 25 % of the



Purification and partial characterization of human lymphoblast interferon.

Preliminary amino-terminal sequencing results support the conclusion that the interferon species is essentially homogeneous.

Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.

It is concluded that human cells contain at least two structural genes for interferon, coding for polypeptides differing in primary sequence.

A bacterial clone synthesizing proinsulin.

We have cloned double-stranded cDNA copies of a rat preproinsulin messenger RNA in Escherichia coli chi1776, using the unique Pst endonuclease site of plasmid pBR322 that lies in the region encoding

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties,

Virus interference. I. The interferon

  • A. IsaacsJ. Lindenmann
  • Medicine
    Proceedings of the Royal Society of London. Series B - Biological Sciences
  • 1957
During a study of the interference produced by heat-inactivated influenza virus with the growth of live virus in fragments of chick chorio-allantoic membrane it was found that following incubation of

Interferon, double-stranded RNA, and protein phosphorylation.

The addition of double-stranded RNA to an extract from Ehrlich ascites tumor cells which have been treated with an interferon preparation promotes the phosphorylation by [gamma-32P]ATP of at least two proteins: P1 and P2.

Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNA

Mouse thymidine kinase-negative L cells were transformed with a cloned rabbit chromosomal β-globin gene linked to the cloned thymazine kinase gene of herpes simplex virus type 1 and produced up to 2,000 copies of rabbit β- globin RNA per cell indistinguishable from its authentic counterpart.

Human leukocyte interferon: production, purification to homogeneity, and initial characterization.

The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol.

Kinetics of the induction of three translation-regulatory enzymes by interferon.

The quantitative measurements of the intracellular level of these enzymes provide a new and convenient model to study the cell's response to interferon and the dose dependence for protein kinase PK-i induction is shown to be similar to that for the development of the antiviral state.