Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

@article{Nagata1980SynthesisIE,
  title={Synthesis in E. coli of a polypeptide with human leukocyte interferon activity},
  author={S. Nagata and H. Taira and A. Hall and L. Johnsrud and M. Streuli and J. Ecs{\"o}di and W. Boll and K. Cantell and C. Weissmann},
  journal={Nature},
  year={1980},
  volume={284},
  pages={316-320}
}
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones. 
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