Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

  title={Synthesis in E. coli of a polypeptide with human leukocyte interferon activity},
  author={S. Nagata and H. Taira and A. Hall and L. Johnsrud and M. Streuli and J. Ecs{\"o}di and W. Boll and K. Cantell and C. Weissmann},
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones. 
Human leukocyte interferon produced by E. coli is biologically active
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli, and protected squirrel monkeys from lethal encephalomyocarditis virus infection. Expand
Structure and expression of human alpha-interferon genes.
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Expression of the human interferon-β gene cloned in phage M13 mp7
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Total synthesis of a human leukocyte interferon gene
A 514-base pair fragment of double-stranded DNA coding for human interferon-α1 (166 amino acid residues), and containing initiation and termination signals plus appropriate restriction enzyme sitesExpand
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Expression of human fibroblast interferon gene in Escherichia coli
Antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized and processed to a size compatible with mature but unglycosylated authentic product. Expand
Construction of expression plasmids producing high levels of human leukocyte-type interferon in Escherichia coli.
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Recombinant DNA Technology and Characterization of Recombinant Alpha-2 Interferon
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Supply of the argU gene product allows high-level expression of recombinant human interferon-a2a in Escherichia coli
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A bacterial clone synthesizing proinsulin.
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Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNA
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Human leukocyte interferon: production, purification to homogeneity, and initial characterization.
The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Expand
Kinetics of the induction of three translation-regulatory enzymes by interferon.
The quantitative measurements of the intracellular level of these enzymes provide a new and convenient model to study the cell's response to interferon and the dose dependence for protein kinase PK-i induction is shown to be similar to that for the development of the antiviral state. Expand