Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

@article{Nagata1980SynthesisIE,
  title={Synthesis in E. coli of a polypeptide with human leukocyte interferon activity},
  author={Shigekazu Nagata and Hideharu Taira and Alan Hall and Lorraine Johnsrud and Michel Streuli and Josef Ecs{\"o}di and Werner Boll and Kari Cantell and Charles Weissmann},
  journal={Nature},
  year={1980},
  volume={284},
  pages={316-320}
}
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones. 

Human leukocyte interferon produced by E. coli is biologically active

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli, and protected squirrel monkeys from lethal encephalomyocarditis virus infection.

Structure and expression of human alpha-interferon genes.

Isolation and structure of a human fibroblast interferon gene

From the nucleotide sequence of the gene, the complete amino acid sequence of human fibroblast interferon was deduced and the protein is 166 amino acids long and is preceded by a 21-amino acid signal sequence.

Total synthesis of a human leukocyte interferon gene

A 514-base pair fragment of double-stranded DNA coding for human interferon-α1 (166 amino acid residues), and containing initiation and termination signals plus appropriate restriction enzyme sites

Expression of human fibroblast interferon gene in Escherichia coli

Antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized and processed to a size compatible with mature but unglycosylated authentic product.

Recombinant DNA Technology and Characterization of Recombinant Alpha-2 Interferon

The strain of E. coli, KMAC-43, used for the large-scale production of alpha-2 interferon was engineered at Schering by Leibowitz and his co-workers. The alpha-2 interferon gene used in this

Supply of the argU gene product allows high-level expression of recombinant human interferon-a2a in Escherichia coli

Escherichia coli BL21 (DE3) co-transformed with the gene of human interferon-α2a (IFN-α2a) and the argU gene that codes for minor tRNA Arg(AGG/AGA) produced IFN-α2a in yield of more than 25 % of the
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    Proceedings of the Royal Society of London. Series B - Biological Sciences
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