Synthesis and utilization of a nonhydrolyzable phosphoadenosine phosphosulfate analog.

@article{Ng1991SynthesisAU,
  title={Synthesis and utilization of a nonhydrolyzable phosphoadenosine phosphosulfate analog.},
  author={K Ng and Mark D'Souza and L Callahan and David H. Geller and A E Kearns and Stephen B. Lyle and Nancy B. Schwartz},
  journal={Analytical biochemistry},
  year={1991},
  volume={198 1},
  pages={
          60-7
        }
}
3'-Phosphoadenosine 5'-phosphosulfate (PAPS) functions as the high-energy sulfate donor for sulfate ester synthesis in all higher organisms. This activated sulfate, like its adenosine 5'-phosphosulfate precursor, is both chemically labile and vulnerable to sulfohydrolase degradation. These obstacles have limited the utility of the native PAPS in the purification and mechanistic description of the numerous PAPS-utilizing enzymes. This paper describes the synthesis of the 2'- and 3'-isomers of a… Expand
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Adenosine-5'-O-(2-fluorodiphosphate) (ADPβF), an analog of adenosine-5'-phosphosulfate
Abstract Adenosine-5′-phosphosulfate (APS) and its 3′-phosphorylated metabolite PAPS (3′-phosphoadenosine-5′-phosphosulfate) play central roles in biological systems. Few analogs of APS or PAPS haveExpand
Synthesis of nucleoside phosphosulfates.
TLDR
Using this method, various NPS with yields ranging from 70-90%, are obtained, including adenosine 5'-phosphosulfate (APS) and 2',3'-cyclic precursor of 3'phosphoadenosine5'-ph phosphate (PAPS), which are the key intermediates in the assimilation and metabolism of sulfur in all living organisms. Expand
Sulfate activation and transport in mammals: system components and mechanisms.
TLDR
Isolation of the PAPS-synthesizing activities from rat chondrosarcoma and preparation of stable non-hydrolyzable analogs of APS and PAPS have facilitated the kinetic characterization of mammalian ATP sulfurylase and APS kinase. Expand

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