Synthesis and utility of 14C-labeled nicotinamide cofactors.

Abstract

A new method for the synthesis of the reduced form of beta-nicotinamide [U-14C]adenine dinucleotide 2(')-phosphate([Ad-14C]NADPH) is presented. The present synthesis results in a radioactive material with a specific activity that is greater than 220 mCi/mmol. This method could easily be adapted for syntheses of 14C-labeled NADH, NADP(+), or any nicotinamide… (More)

Topics

2 Figures and Tables

Cite this paper

@article{Markham2004SynthesisAU, title={Synthesis and utility of 14C-labeled nicotinamide cofactors.}, author={Kelli A. Markham and R Steven Sikorski and Amnon Kohen}, journal={Analytical biochemistry}, year={2004}, volume={325 1}, pages={62-7} }