Synthesis and trafficking of prion proteins in cultured cells.

  title={Synthesis and trafficking of prion proteins in cultured cells.},
  author={Albert Taraboulos and Alex J. Raeber and David R Borchelt and D Serban and Stanley B Prusiner},
  journal={Molecular biology of the cell},
  volume={3 8},
Scrapie prions are composed largely, if not entirely, of the scrapie prion protein (PrPSc) that is encoded by a chromosomal gene. Scrapie-infected mouse neuroblastoma (ScN2a) and hamster brain (ScHaB) cells synthesize PrPSc from the normal PrP isoform (PrPC) or a precursor through a posttranslational process. In pulse-chase radiolabeling experiments, we found that presence of brefeldin A (BFA) during both the pulse and the chase periods prevented the synthesis of PrPSc. Removal of BFA after the… CONTINUE READING

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