Synthesis and secretion of mucin by the human colonic tumour cell line LS180.

@article{McCool1994SynthesisAS,
  title={Synthesis and secretion of mucin by the human colonic tumour cell line LS180.},
  author={Doris J. McCool and Janet F. Forstner and Gordon G. Forstner},
  journal={The Biochemical journal},
  year={1994},
  volume={302 ( Pt 1)},
  pages={111-8}
}
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each time point, mucin proteins were immunoprecipitated with specific anti-mucin antibodies and analysed by SDS/PAGE and fluorography. At short labelling times with [3H]threonine, without chase, a monomeric thiol… CONTINUE READING