Synthesis and secretion of a 38-kDa glycopolypeptide coincides with L6 myoblast fusion.

Abstract

Rat L6 myoblastic cell line fused rapidly after two day cultivation in a medium containing horse serum and insulin. We analyzed whether the induction of plasma membrane or secreted proteins occurred simultaneously with ongoing fusion. Thus the cells were metabolically labeled with [35S]methionine followed by biotinylation of the cell surface proteins… (More)

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