Synthesis and purification of oligonucleotides.

  title={Synthesis and purification of oligonucleotides.},
  author={Andrew D. Ellington and Jane D. Pollard},
  journal={Current protocols in molecular biology},
  volume={Chapter 2},
This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.