Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.

Abstract

Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission characteristics are well retained. Their cell-permeable esters have been prepared for intracellular applications.

Cite this paper

@article{Liu2001SynthesisAP, title={Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.}, author={Jun Liu and Zhenjun Diwu and Wai Y Leung}, journal={Bioorganic & medicinal chemistry letters}, year={2001}, volume={11 22}, pages={2903-5} }