Synthesis and performance of megaporous immobilized metal-ion affinity cryogels for recombinant protein capture and purification.

Megaporous cryogels with metal-ion affinity functionality, which possess enhanced protein-binding ability, were synthesized and their properties were investigated. These highly porous materials (pore sizes up to 100 μm) allowed the direct capture of a recombinant His(6)-tagged protein from a partially clarified extract. The total ligand density of the… CONTINUE READING