Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes.

  title={Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes.},
  author={S. Bar-Nun and G. Kreibich and M. Adesnik and L. Alterman and M. Negishi and D. Sabatini},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={77 2},
Treatment of rats with phenobarbital leads to a substantial increase in levels of translatable liver cytochrome P-450 mRNA. This mRNA is primarily associated with ribosomes bound to endoplasmic reticulum membranes which in an in vitro system synthesized approximately 10 times more cytochrome P-450 than did free polysomes from the same animals. Cytochrome P-450 synthesized by rough microsomes in vitro appears to be directly inserted into the membranes because it was not released by a treatment… Expand
Cytochrome P-450 in the endoplasmic reticulum. Biosynthesis, distribution, induction, and degradation.
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Determination of the membrane topology of the phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4 using site-specific antibodies
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