Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes.

@article{BarNun1980SynthesisAI,
  title={Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes.},
  author={S. Bar-Nun and G. Kreibich and M. Adesnik and L. Alterman and M. Negishi and D. Sabatini},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 2},
  pages={
          965-9
        }
}
Treatment of rats with phenobarbital leads to a substantial increase in levels of translatable liver cytochrome P-450 mRNA. This mRNA is primarily associated with ribosomes bound to endoplasmic reticulum membranes which in an in vitro system synthesized approximately 10 times more cytochrome P-450 than did free polysomes from the same animals. Cytochrome P-450 synthesized by rough microsomes in vitro appears to be directly inserted into the membranes because it was not released by a treatment… Expand
Cytochrome P-450 in the endoplasmic reticulum. Biosynthesis, distribution, induction, and degradation.
TLDR
P-450 forms function in the metabolism of a wide variety of exogenous compounds such as drugs and chemical carcinogens and endogenous ones such as steroids, fatty acids, and retinoids and have been characterized by their specific, but overlapping, substrate specificities and by their inducibility. Expand
Studies on the biosynthesis of microsomal membrane proteins. Site of synthesis and mode of insertion of cytochrome b5, cytochrome b5 reductase, cytochrome P-450 reductase and epoxide hydrolase.
TLDR
Mechanisms which may account for the spatial disposition of the membrane proteins and for their selective accumulation in endoplasmic reticulum membranes are discussed. Expand
Critical amino-terminal segments in insertion of rat liver cytochrome P450 3A1 into the endoplasmic reticulum membrane
TLDR
The membrane topogenesis of rat liver cytochrome P450 3A1 is determined mainly by the amino-terminal hydrophobic segment, and proteolysis-resistant membrane-associated peptides were observed in all the in vitro translated mutants containing that segment. Expand
Signals for the incorporation and orientation of cytochrome P450 in the endoplasmic reticulum membrane
TLDR
The extreme hydrophobicity of the amino-terminal portion of P450b suggests that it not only serves to initiate the cotranslational insertion of the nascent polypeptide but that it also halts translocation of downstream portions into the lumen of the ER and anchors the mature protein in the membrane. Expand
Synthesis and insertion, both in vivo and in vitro, of rat-liver cytochrome P-450 and epoxide hydratase into Xenopus laevis membranes.
TLDR
Systems capable of inserting into membranes cytochrome P-450 and epoxide hydratase made under the direction of rat liver RNA and cell-free systems used to study the pathways followed by newly made secretory and integral membrane proteins appear to involve a common initial pathway. Expand
The induction of microsomal electron transport enzymes
TLDR
The present review summarizes the historic background leading to current concepts of cy tochrome P-450 induction and describes recent advances in the knowledge of the regulation of cytochromeP-450 synthesis in the liver. Expand
Biosynthesis of lysosomal hydrolases: their synthesis in bound polysomes and the role of co- and post-translational processing in determining their subcellular distribution
TLDR
The observations suggest that the hydrolases exported into the medium follow the normal secretory route and that some of their oligosaccharides are subject to modifications known to affect many secretory glycoproteins during their passage through the Golgi apparatus. Expand
Biosynthesis Ribophorins and Processing of in the Endoplasmic Reticulum
Ribophorins are two transmembrane glycoproteins characteristic of the rough endoplasmic reticulum, which are thought to be involved in the binding of ribosomes. Their biosynthesis was studied in vivoExpand
The processing and assembly of rat liver MAO
In eukaryotic cells enzymes are specifically compartmentalized either in intracellular membranes or in the spaces enclosed by these membranes. While the processes involved in the distribution ofExpand
Determination of the membrane topology of the phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4 using site-specific antibodies
TLDR
Results favor a model in which the cytochrome P-450 molecule is largely exposed on the cytoplasmic surface of the endoplasmic reticulum membrane to which it is anchored by its short amino terminal hydrophobic segment. Expand
...
1
2
3
4
5
...