Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. Response to the removal of extracellular cartilage matrix

@article{Dessau1978SynthesisAE,
  title={Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. Response to the removal of extracellular cartilage matrix},
  author={Waltraud Dessau and Joachim Sasse and Rupert Timpl and Franti{\vs}ek J{\'i}lek and Klaus von der Mark},
  journal={The Journal of Cell Biology},
  year={1978},
  volume={79},
  pages={342 - 355}
}
Fibronectin, the major cell surface glycoprotein of fibroblasts, is absent from differentiated cartilage matrix and chondrocytes in situ. However, dissociation of embryonic chick sternal cartilage with collagenase and trypsin, followed by inoculation in vitro reinitiates fibronectin synthesis by chondrocytes. Immunofluorescence microscopy with antibodies prepared against plasma fibronectin (cold insoluble globulin [CIG]) reveals fibronectin associated with the chondrocyte surface. Synthesis and… CONTINUE READING