Synthesis and evaluation of fluorescent probes for the detection of calpain activity.

@article{Mittoo2003SynthesisAE,
  title={Synthesis and evaluation of fluorescent probes for the detection of calpain activity.},
  author={Stifun Mittoo and Lars E. Sundstrom and Mark Jones Bradley},
  journal={Analytical biochemistry},
  year={2003},
  volume={319 2},
  pages={234-8}
}
Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in alpha-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol… CONTINUE READING

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