Synthesis and characterization of the dansyltyrosine derivatives of porcine pancreatic colipase.

@article{McIntyre1990SynthesisAC,
  title={Synthesis and characterization of the dansyltyrosine derivatives of porcine pancreatic colipase.},
  author={J C McIntyre and Friedhelm Schroeder and W. David Behnke},
  journal={Biochemistry},
  year={1990},
  volume={29 8},
  pages={
          2092-101
        }
}
Steady-state and time-resolved fluorescence techniques were used to study dansyltyrosine derivatives of porcine pancreatic colipase. Nitration, reduction, acylation, and dansylation reactions were utilized to synthesize two fluorescently labeled colipases: (o-aminodansyltyrosine 55 porcine colipase) (DNStyr55PC) and o-aminodansyltyrosine 59 porcine colipase (DNStyr59PC). DNStyr55PC was 200% active, while the DNStyr59 derivative maintained 80% activity in a pH stat assay. Emission spectra… 
The interaction of bile salt micelles with the dansyltyrosine derivatives of porcine colipase.
The interaction of bile salt micelles with the tyrosines of pancreatic colipase was assessed by steady-state and time-resolved fluorescence techniques. Dansyltyrosine fluorescence showed that Tyr-55
Identification of amino acids in human colipase that mediate adsorption to lipid emulsions and mixed micelles.
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Contrary to the predictions of computer modeling, the results provide strong support for an essential role of Tyr 55 in colipase adsorption to mixed micelles.
Neurophysin-neurohypophyseal hormone interactions: studies using a dansylated vasotocin analogue.
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A neurohypophyseal hormone analogue containing an extrinsic fluorescence probe is synthesized by linking a dansyl (DNS) group to the epsilon-amino group of the lysine at residue 8 of vasotocin using steady-state and time-resolved spectroscopic methods.
Critical Role of Micelles in Pancreatic Lipase Activation Revealed by Small Angle Neutron Scattering*
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It is shown that either bile salts, lysophospholipids, or nonionic detergents that form micelles with radii of gyration ranging from 13 to 26 Å are able to bind to the PC-PL complex, whereas smaller micells or nonmicellar compounds are not, which further supports the notion of a micelle size-dependent affinity process for lipase activationin vivo.
Crystallographic study of the structure of colipase and of the interaction with pancreatic lipase
TLDR
Colipase lacks well‐defined secondary structure elements and seems to be stabilized mainly by an extended network of five disulfide bridges that runs throughout the flatly shaped molecule, reticulating its four finger‐like loops.
Fluorescence probes in biochemistry: an examination of the non-fluorescent behavior of dansylamide by photoacoustic calorimetry.
TLDR
Photoacoustic calorimetry combined with established absorption and fluorescence methodologies provides a complete arsenal for characterizing the photophysical properties of many systems.
A rapid and selective mass spectrometric method for the identification of nitrated proteins.
TLDR
A totally new approach involving dansyl chloride labeling of the nitration sites which relies on the enormous potential of MS(n) analysis is reported which was successfully applied to the identification of 3-nitrotyrosine residues in complex protein mixtures.
Fluorescence assay for phospholipid membrane asymmetry.
TLDR
It is demonstrated how reduction of NBD-lipids with dithionite can be used to prepare asymmetrically labeled liposomes and to measure transverse-membrane asymmetry in vesicles and the measurement of phospholipid translocase activity.
Interactions of bile salt micelles and colipase studied through intermolecular nOes
TLDR
Investigating the organization of sodium taurodeoxycholate (NaTDC) micelles and their interactions with pig and horse colipases by homonuclear nuclear magnetic resonance (NMR) spectroscopy supply evidence that the folding of horse Colipase is similar to that already described for pig colipase.
Influence of various salts on the reversed-phase retention of some dansylated amino acids in TLC
SummaryThe retention of 14 dansylated amino acid derivatives was determined using aqueous LiCl, NaCl, KCl, RbCl and CsCl solutions as eluents in reversed-phase thinlayer chromatography. The salts
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TLDR
The nitration of the long form (N-terminal valine) of porcine pancreatic colipase with tetranitromethane was investigated under a variety of conditions and data suggest that Tyr-58 and Tyr-59 are part of the interface recognition site ofcolipase.
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TLDR
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