Synthesis and characterization of oligonucleotides containing formamidopyrimidine lesions (Fapy.dA, Fapy.dG) at defined sites.

@article{Haraguchi2001SynthesisAC,
  title={Synthesis and characterization of oligonucleotides containing formamidopyrimidine lesions (Fapy.dA, Fapy.dG) at defined sites.},
  author={Kazuhiro Haraguchi and Michael Oren Delaney and Carissa J. Wiederholt and Aruna Sambandam and Zsolt Hantosi and Marc M Greenberg},
  journal={Nucleic acids research. Supplement},
  year={2001},
  volume={1},
  pages={
          129-30
        }
}
The preparation of oligonucleotides containing Fapy.dA (N4-(2-Deoxy-alpha,beta-D-erythro-pentofuranosyl)-4,6-diamino- 5-formamidopyrimidine) and Fapy.dG (N6-(2-Deoxy-alpha,beta-D-erythro-pento-furanosyl)-2,6- diamino-4-hydroxy-5-formamido-pyrimidine) at defined sites was achieved by introducing the lesions as dinucleotide phosphoramidites. Oligonucleotides as composed of as many as 36-nucleotides were prepared by solid-phase synthesis and/or a combination of chemical synthesis and enzymatic… CONTINUE READING
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