Synthesis and characterization of mu-conotoxin IIIa.

  title={Synthesis and characterization of mu-conotoxin IIIa.},
  author={Susanne Becker and Eric Atherton and Robert D Gordon},
  journal={European journal of biochemistry},
  volume={185 1},
mu-Conotoxin IIIa, a voltage-dependent sodium channel neurotoxin, has been synthesised using solid-phase peptide synthesis employing 9-fluorenylmethoxycarbonyl chemistry. After cleavage from the resin, the peptide was isolated by reverse-phase HPLC and then the six acetamidomethyl groups were removed by treatment with mercuric acetate. The reduced product so formed was purified by reverse-phase HPLC. Protocols were developed to optimize the oxidation of the cysteine residues to form disulphide… CONTINUE READING