Synthesis and characterization of chimeric silkworm silk.

@article{Asakura2003SynthesisAC,
  title={Synthesis and characterization of chimeric silkworm silk.},
  author={Tetsuo Asakura and Koji Nitta and Mingying Yang and Juming Yao and Yasumoto Nakazawa and David L Kaplan},
  journal={Biomacromolecules},
  year={2003},
  volume={4 3},
  pages={815-20}
}
A synthetic gene encoding a chimeric silklike protein was constructed that combined a polyalanine encoding region (Ala)(18), a sequence slightly longer than the (Ala)(12-13) found in the silk fibroin from the wild silkworm Samia cynthia ricini, and a sequence encoding GVGAGYGAGAGYGVGAGYGAGVGYGAGAGY, found in the silk fibroin from the silkworm Bombyx mori. A tetramer of the chimeric repeat sequence encoding a approximately 29 kDa protein was expressed as a fusion protein in Escherichia coli. In… CONTINUE READING