Synthesis and back exchange of 18O labeled amino acids for use as internal standards with mass spectrometry.

@article{Murphy1979SynthesisAB,
  title={Synthesis and back exchange of 18O labeled amino acids for use as internal standards with mass spectrometry.},
  author={Robert C. Murphy and Keith L. Clay},
  journal={Biomedical mass spectrometry},
  year={1979},
  volume={6 7},
  pages={309-14}
}
Amino acids with two oxygen-18 atoms in the carboxyl moiety can be synthesized easily from the amino acids and H2 18O through exchange. The exchange conditions are simply solution of the amino acid (1-4 mg) in acidic H2 18O (0.1-0.2ml) followed by heating at 60-70 degrees C for several days. Eighteen oxygen-18 amino acids with incorporations typically… CONTINUE READING