Synthesis and antituberculosis activity of new thiazolylhydrazone derivatives.

Abstract

The increasing clinical importance of drug-resistant mycobacterial pathogens has lent additional urgency to microbiological research and new antimycobacterial compound development. For this purpose, new thiazolylhydrazone derivatives were synthesized and evaluated for antituberculosis activity. The reaction of thiosemicarbazide with acetophenone derivatives gave 1-(1-arylethylidene)thiosemicarbazide (1). The N-(1-arylethylidene)-N'-[4-(indan-5-yl)thiazol-2-yl]hydrazone (3) derivatives were synthesized by reacting 1-(1-arylethylidene)thiosemicarbazide with 1-(5-indanyl)-2-bromoethanone (2). The chemical structure of the compounds was elucidated by elemental analyses, IR, (1)H NMR, MS-FAB(+) spectral data. Antituberculosis activities of the synthesized compounds were determined by broth microdilution assay, the Microplate Alamar Blue Assay, in BACTEC12B medium and the results were screened in vitro, using BACTEC 460 Radiometric System against Mycobacterium tuberculosis H(37)Rv (ATCC 27294) at 6.25 microg/ml and some of the tested compounds showed important inhibition ranging from 92% to 96%. The compounds were also investigated for their cytotoxic properties on normal mouse fibroblast (NIH/3T3) cell line and the results obtained here showed that all the compounds used have no significant cytotoxicity at the concentrations under 50 microg/ml.

Cite this paper

@article{TuranZitouni2008SynthesisAA, title={Synthesis and antituberculosis activity of new thiazolylhydrazone derivatives.}, author={G{\"{u}lhan Turan-Zitouni and Ahmet Refik Ozdemir and Zafer Asim Kaplancikli and Kadriye Benkli and Pierre Chevallet and Gulsen Akalin}, journal={European journal of medicinal chemistry}, year={2008}, volume={43 5}, pages={981-5} }