Synthesis and anticoagulant activity of heparin immobilized "end-on" to polystyrene microspheres coated with end-group activated polyethylene oxide.

@article{Fry2010SynthesisAA,
  title={Synthesis and anticoagulant activity of heparin immobilized "end-on" to polystyrene microspheres coated with end-group activated polyethylene oxide.},
  author={Allyson Kaye Fry and Karl F Schilke and Joseph S. Mcguire and Karyn E. Bird},
  journal={Journal of biomedical materials research. Part B, Applied biomaterials},
  year={2010},
  volume={94 1},
  pages={187-95}
}
Thiol groups were introduced to unfractionated heparin (UFH) and end-aminated heparin (HepNH(2)) by reaction with 2-iminothiolane under conditions favoring selective modification of terminal over primary amines. End-thiolated heparin retained anticoagulant activity as shown by the activated partial thromboplastin time (aPTT) and anti-Factor Xa (anti-FXa) assays. Thiolated heparins were linked to pyridyl-disulfide activated poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock… CONTINUE READING