Synthesis, penetrability and intracellular targeting of fluorescein-tagged peptoids and peptide-peptoid hybrids.

@article{UncitiBroceta2009SynthesisPA,
  title={Synthesis, penetrability and intracellular targeting of fluorescein-tagged peptoids and peptide-peptoid hybrids.},
  author={Asier Unciti-Broceta and Franziska Diezmann and Chiung Ying Ou-Yang and Mario Antonio Fara and Mark Bradley},
  journal={Bioorganic & medicinal chemistry},
  year={2009},
  volume={17 3},
  pages={959-66}
}
The search for novel, generally applicable and highly efficient delivery tools is a major activity in the biotechnology arena. Using highly optimized microwave based solid-phase chemistry a series of fluorescein-labelled cationic peptoid conjugates were synthesized within 24h and cellular uptake into HeLa, L929 and K562 cells examined via flow cytometry. As expected, analysis revealed that longer oligomers achieved greater cellular penetration (7e (9 mer)>7d (7 mer)>7c (5 mer)>7b (3 mer)>7a (1… CONTINUE READING