Synergy between tetA and rpsL provides high-stringency positive and negative selection in bacterial artificial chromosome vectors.

@article{Stavropoulos2001SynergyBT,
  title={Synergy between tetA and rpsL provides high-stringency positive and negative selection in bacterial artificial chromosome vectors.},
  author={Tom A. Stavropoulos and Craig A. Strathdee},
  journal={Genomics},
  year={2001},
  volume={72 1},
  pages={99-104}
}
Bacterial artificial chromosome (bacmid) vectors are used to stably propagate large, complex fragments of cloned DNA and are a core technology for functional genomics. The simplest method of analyzing bacmid clones would involve a direct mutagenesis or allele exchange protocol utilizing positive and negative selectable markers. The utility of three different negative selectable markers to function in the context of a bacmid vector was therefore investigated: sacB from Bacillus subtilis, which… CONTINUE READING
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