Synergistic toxicity of iron and arachidonic acid in HepG2 cells overexpressing CYP2E1.

@article{Caro2001SynergisticTO,
  title={Synergistic toxicity of iron and arachidonic acid in HepG2 cells overexpressing CYP2E1.},
  author={Andr{\'e}s Caro and Arthur I. Cederbaum},
  journal={Molecular pharmacology},
  year={2001},
  volume={60 4},
  pages={742-52}
}
Priming of the liver for ethanol-induced injury, by nutrients such as polyunsaturated fat and iron, plays a key role in alcoholic liver disease. The objective of this work was to evaluate the effect of the combination of Fe-nitrilotriacetic acid (Fe-NTA) and arachidonic acid (AA) on the viability of HepG2 cells (E47 cells) transfected to express human CYP2E1. Cells were plated, preloaded with arachidonic acid, washed, and exposed to Fe-NTA for variable periods. Fe-NTA (10 microM) or AA (5… CONTINUE READING

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CYP 2 E 1 and

  • H Tsukamoto
  • ALD Hepatology
  • 2000
2 Excerpts

ethanol-induced cellular injury

  • JG Pastorino, JB Hoek
  • Free Radic Biol Med
  • 2000
2 Excerpts

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