Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytes.

@article{Stoll2012SynergisticEO,
  title={Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytes.},
  author={Christoph Stoll and Jelena Lecak Holovati and Jason P Acker and Willem F Wolkers},
  journal={Biotechnology progress},
  year={2012},
  volume={28 2},
  pages={364-71}
}
Red blood cells (RBCs) can be cryopreserved using glycerol as a cryoprotective agent, but one of the main disadvantages is the time-consuming deglycerolization step. Novel cryopreservation strategies for RBCs using nontoxic cryoprotective agents are urgently needed. The effect of DMPC, DOPC, and DPPC liposomes on survival of RBCs cryopreserved with trehalose and HES has been evaluated. DMPC caused hemolysis before freezing and affected RBC deformability parameters. DMPC treated RBCs displayed a… CONTINUE READING
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