Synergistic Effects of Copper Sites on Apparent Stability of Multicopper Oxidase, Fet3p

@inproceedings{Sedlk2018SynergisticEO,
  title={Synergistic Effects of Copper Sites on Apparent Stability of Multicopper Oxidase, Fet3p},
  author={Erik Sedl{\'a}k and Gabriel Žold{\'a}k and Pernilla Wittung-Stafshede},
  booktitle={International journal of molecular sciences},
  year={2018}
}
Saccharomyces cerevisiae Fet3p is a multicopper oxidase that contains three cupredoxin-like domains and four copper ions located in three distinct metal sites (T1 in domain 3; T2 and the binuclear T3 at the interface between domains 1 and 3). To probe the role of the copper sites in Fet3p thermodynamic stability, we performed urea-induced unfolding experiments with holo-, apo- and three partially-metallated (T1, T2 and T1/T2 sites depleted of copper) forms of Fet3p. Using a combination of… CONTINUE READING

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